human escc cell line ec109  (AddexBio Inc)

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: The expression of miR-125b in ESCC tissues and cells. (A) The expression of miR-125b in ESCC and paired normal tissues was examined by qRT-PCR. (B) The expression of miR-125b in ESCC cell lines and a human esophageal epithelial cell line was examined by qRT-PCR. *P<0.05 vs. the control. ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: The association between the expression level of miR-125b with the clinical characteristics of ESCC patients.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Expressing

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: miR-125b inhibits ESCC cell proliferation. (A) EC109 and EC9706 cells transfected with miR-125b mimics or miR-125b inhibitors significantly increased or decreased, respectively, the expression of miR-125b compared with the control. (B) EC109 and EC9706 cells transfected with miR-125b mimics or miR-125b inhibitors both exhibited a significant decrease or increase, respectively, in the proliferation rate compared with the control. *P<0.05 vs. the control. ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Transfection, Expressing, Control

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: miR-125b inhibits the ESCC cell cycle. EC109 and EC9706 cells transfected with miR-125b mimics significantly increased the G1 phase of the cell cycle compared with the control. *P<0.05 vs. the control. ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Transfection, Control

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: miR-125b induces ESCC cell apoptosis. (A) EC109 and EC9706 cells transfected with miR-125b mimics significantly increased cell apoptosis compared with the control. (B) The expression of caspase-3, Bcl-2, Bax and p27 were analyzed in ESCC cell lines. *P<0.05 vs. the control. ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Transfection, Control, Expressing

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: BMF is a direct target of miR-125b in ESCC cancer cells. (A) The prediction of the binding between miR-125b and BMF as determined using TargetScan. (B) A dual-luciferase reporter assay was performed to verify the binding of miR-125b with BMF. (C) qRT-PCR assay was performed to detect the mRNA level of BMF in EC109 and EC9706 cells treated with miR-125b mimics and miR-125b inhibitors. (D) The expression of BMF was assessed in the tumor sections. *P<0.05 vs. the control. BMF, BCL-2-modifying factor; ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Binding Assay, Luciferase, Reporter Assay, Quantitative RT-PCR, Expressing, Control

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: BMF inhibits ESCC cell proliferation. (A) A qRT-PCR assay was conducted to assess the mRNA expression of BMF. (B) Western blot analysis was performed to assess the protein expression of BMF. (C) A CCK-8 assay was used to reveal the proliferation rate in ESCC cells with si-BMF transfection. (D) The cell cycle was examined in ESCC cell lines. *P<0.05 vs. the control. BMF, BCL-2-modifying factor; ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, CCK-8 Assay, Transfection, Control

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: BMF induces ESCC cell apoptosis. (A) Cell apoptosis was assayed in ESCC cell lines. (B) The protein level was assayed by western blotting in ESCC cell lines *P<0.05 vs. the control. BMF, BCL-2-modifying factor; ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Western Blot, Control

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: Relationship between miR-125b and BMF in ESCC. (A) The mRNA expression of BMF in ESCC tissues compared to normal tissues. (B) The mRNA expression of BMF in ESCC cell lines (EC109 and EC9706 cells) compared to an esophageal epithelial cell line (HET-1A). (C) The protein expression of BMF in ESCC tissues compared to normal tissues. (D) The protein expression of BMF in ESCC cells (EC109 and EC9706 cells) compared to an esophageal epithelial cell line (HET-1A). (E) Data analysis of relationship between the expression of miR-125b and BMF in ESCC tissues. *P<0.05 vs. the control. BMF, BCL-2-modifying factor; ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Expressing, Control